产品：人粒细胞集落刺激因子酶联免疫试剂盒

货号：EK0360

Description

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoproteins that regulates hematopoietic cell proliferation and differentiation. In semi-solid agar culture, it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells. G-CSF is also known as colony stimulating factor-3, and a single gene of it codes for a 177 or 180 amino acid mature protein with a molecular weight 19,600. Functionally, it stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Additionally, it prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. The human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.

ScienCells human G-CSF ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human G-CSF specific polyclonal antibody is pre-coated onto 8 x 12 divisible strips. The human G-CSF specific detection polyclonal antibody is biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates re washed away with TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human G-CSF captured in strips.

Product Use

This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

Storage

Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.

Shipping

Shipped on gel ice.

Reference

1. Nagata S, Tsuchiya M, Asano S, Kaziro Y, Yamazaki T, Yamamoto O, Hirata Y, Kubota N, Oheda M, Nomura H, et al. Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor. Nature 1986 Jan 30-Feb 5; 319 (6052), 415-8.
2. Harada M, Qin Y, Takano H, Minamino T, Zou Y, Toko H, Ohtsuka M, Matsuura K, Sano M, Nishi J, Iwanaga K,Akazawa H, Kunieda T, Zhu W, Hasegawa H, Kunisada K, Nagai T, Nakaya H, Yamauchi-Takihara K, Komuro I.G-CSF prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes.Nat Med 2005 Mar; 11 (3):305-11. 2005 Feb 20.
3. Kanda N, Fukushige S, Murotsu T, Yoshida MC, Tsuchiya M, Asano S, Kaziro Y, Nagata S. Human gene coding for granulocyte-colony stimulating factor is assigned to the q21-q22 region of chromosome 17. Somat Cell Mol Genet1987 Nov; 13 (6):679-84

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 Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应 

       近几年的新药研发成功率在逐年下降，其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利用这些原代细胞株所甄别和筛选出来的候选药物，其诊治人类疾病的成功几率将大大增加。这样不但大大节省了新药的开发成本，而且将极大地提高人类的健康质量。这些产品从根本上提高了全球生命医学研究、人类重要疾病药物研发、新药研发的成功率。所以上海中乔新舟生物科技有限公司致力于提供优质原代细胞产品和完善的售后服务。 
     
       美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。

        ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。 确保了实验结果的真实性、重复性和连贯性。

Sciencell公司部分原代细胞目录（如需要其他细胞资料请发邮件至 wwwfudan@163.com索取）： 
       
        中乔新舟www.zqxzbio.com专长于为生物医药领域的医疗机构、研究中心、企业、临床医生等提供课题设计、基金联合申请、实验技术服务、论文相关服务、采购外包等整体服务，目前已经成长为国内领先的转化医学外包品牌。
         中乔新舟的PI团队已经发展到专职PI 20人，联席PI 312人，其中大多数拥有海外背景。截止到2009年3月，以PI或联席PI为第一作者发表SCI 论文1682 篇（总IF 值为5721.82，其中影响因子IF>5.0 的论文216 篇，IF>10.0论文32 篇）。
  
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